catalytic subunit (New England Biolabs)
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New England Biolabs
catalytic subunit
Catalytic Subunit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pka+catalytic+subunit/cAMP-dependent+Protein+Kinase+(PKA)%2C+catalytic+subunit/10__1016_slash_j__snb__2026__141050-49-1-13
Average 95 stars, based on 772 article reviews
Catalytic Subunit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pka+catalytic+subunit/cAMP-dependent+Protein+Kinase+(PKA)%2C+catalytic+subunit/10__1016_slash_j__snb__2026__141050-49-1-13
Average 95 stars, based on 772 article reviews
catalytic subunit - by Bioz Stars,
2026-11
95/100 stars
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Related Articles
Purification:Article Title: Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Article Snippet: .. Purified biosensors (1 μ M) were incubated with 5 μ g of Article Title: PKAc-directed interaction and phosphorylation of Ptc is required for Hh signaling inhibition in Drosophila Article Snippet: .. 175 In vitro phosphorylation kinase assay using ATP analog ATPγS 176 Prepared the purified substrates and reaction mixture as follows: 10x PKA Reaction 177 Buffer (New England Biolabs), Incubation:Article Title: Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Article Snippet: .. Purified biosensors (1 μ M) were incubated with 5 μ g of Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation. Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl2, 200 μM ATP, and pH 7.5) with 0.5 U/μL Article Title: Phosphorylation on Ser-359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses Article Snippet: .. To detect phosphorylation by PKA, GST-α2 WT and GST-α2 S359A immobilized on GSH-Sepharose beads were incubated (30 °C, 30 min) with 0.75 μg of Article Title: PKAc-directed interaction and phosphorylation of Ptc is required for Hh signaling inhibition in Drosophila Article Snippet: .. 175 In vitro phosphorylation kinase assay using ATP analog ATPγS 176 Prepared the purified substrates and reaction mixture as follows: 10x PKA Reaction 177 Buffer (New England Biolabs), Article Title: Phosphorylation on Ser-359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses Article Snippet: .. To detect phosphorylation by PKA, GST-α2 WT and GSTα2 S359A immobilised on glutathione sepharose beads were incubated (30°C, 30 min) with 0.75 μg Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl 2 , 200 μM ATP, and pH 7.5) with 0.5 U/μL Kinase Assay:Article Title: Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Article Snippet: .. Purified biosensors (1 μ M) were incubated with 5 μ g of Article Title: PKAc-directed interaction and phosphorylation of Ptc is required for Hh signaling inhibition in Drosophila Article Snippet: .. 175 In vitro phosphorylation kinase assay using ATP analog ATPγS 176 Prepared the purified substrates and reaction mixture as follows: 10x PKA Reaction 177 Buffer (New England Biolabs), Blocking Assay:Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation. Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl2, 200 μM ATP, and pH 7.5) with 0.5 U/μL Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl 2 , 200 μM ATP, and pH 7.5) with 0.5 U/μL Phospho-proteomics:Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation. Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl2, 200 μM ATP, and pH 7.5) with 0.5 U/μL Article Title: Phosphorylation on Ser-359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses Article Snippet: .. To detect phosphorylation by PKA, GST-α2 WT and GST-α2 S359A immobilized on GSH-Sepharose beads were incubated (30 °C, 30 min) with 0.75 μg of Article Title: PKAc-directed interaction and phosphorylation of Ptc is required for Hh signaling inhibition in Drosophila Article Snippet: .. 175 In vitro phosphorylation kinase assay using ATP analog ATPγS 176 Prepared the purified substrates and reaction mixture as follows: 10x PKA Reaction 177 Buffer (New England Biolabs), Article Title: Phosphorylation on Ser-359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses Article Snippet: .. To detect phosphorylation by PKA, GST-α2 WT and GSTα2 S359A immobilised on glutathione sepharose beads were incubated (30°C, 30 min) with 0.75 μg Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl 2 , 200 μM ATP, and pH 7.5) with 0.5 U/μL Membrane:Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation. Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl2, 200 μM ATP, and pH 7.5) with 0.5 U/μL Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl 2 , 200 μM ATP, and pH 7.5) with 0.5 U/μL In Vitro:Article Title: PKAc-directed interaction and phosphorylation of Ptc is required for Hh signaling inhibition in Drosophila Article Snippet: .. 175 In vitro phosphorylation kinase assay using ATP analog ATPγS 176 Prepared the purified substrates and reaction mixture as follows: 10x PKA Reaction 177 Buffer (New England Biolabs), other:Article Title: Comparing ATPase activity of ATP-binding cassette subfamily C member 4, lamprey CFTR, and human CFTR using an antimony-phosphomolybdate assay Article Snippet: The following solutions were mixed sequentially: Buffer 2, Transcranial Magnetic Stimulation:Article Title: Phospholamban pentamerization increases sensitivity and dynamic range of cardiac relaxation Article Snippet: .. Membranes were incubated in AC buffer [10% glycerol, 100 mM NaCl, 20 mM Tris (pH 7.5), 1 mM EDTA, 0.1% Tween-20, 2% milk powder, and 1 mM DTT] for 45 min before blocking with TBS-T containing 5% milk for 1 h. PKA phosphorylation of membrane-bound protein was carried out at 30°C in PKA reaction buffer (50 mM HEPES, 10 mM MgCl 2 , 200 μM ATP, and pH 7.5) with 0.5 U/μL |